Recent publications


A cascade of arabinosyltransferases controls shoot meristem size in tomato. Xu C, Liberatore KL, MacAlister CA, Huang Z, Chu YH, Jiang K, Brooks C, Ogawa-Ohnishi M, Xiong G, Pauly M, Van Eck J, Matsubayashi Y, van der Knaap E, Lippman ZB. Nat Genet. [Epub ahead of print]

Arabidopsis leucine-rich repeat extensin (LRX) proteins modify cell wall composition and influence plant growth. Draeger C, Ndinyanka Fabrice T, Gineau E, Mouille G, Kuhn BM, Moller I, Abdou MT, Frey B, Pauly M, Bacic A, Ringli C. BMC Plant Biol. 15:155.

Engineering of plant cell walls for enhanced biofuel production. Loqué D, Scheller HV, Pauly M. Curr Opin Plant Biol. 3;25:151-161.

The presence of fucogalactoxyloglucan and its synthesis in rice indicates conserved functional importance in plants. Liu L, Paulitz J, Pauly M. Plant Physiology 168(2):549-60.

Nitric-acid hydrolysis of miscanthus giganteus to fermentable sugars. Yang F, Afzal W, Cheng K, Liu N, Pauly M, Bell AT, Liu Z, Prausnitz JM. Biotechnology and Bioprocess Engineering [In press]

Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis. Ebert B, Rautengarten C, Guo X, Xiong G, Stonebloom S, Smith-Moritz AM, Herter T, Chan LJ, Adams PD, Petzold CJ, Pauly M, Willats WG, Heazlewood JL, Scheller HV. Plant Cell 27(4):1218-27.

Glucuronic acid moieties on xylan are functionally equivalent to O-acetyl-substituents. Xiong G, Dama M, Pauly M. Mol Plant [Epub ahead of print]

The role of the plant-specific AXY9 protein in Arabidopsis cell wall polysaccharide O-acetylation. Schultink A, Naylor D, Dama M, Pauly M. Plant Physiology 167(4):1271-83.

An Arabidopsis gene regulatory network for secondary cell wall synthesis.
Taylor-Teeples M, Lin L, de Lucas M, Turco G, Toal TW, Gaudinier A, Young NF, Trabucco GM, Veling MT, Lamothe R, Handakumbura PP, Xiong G, Wang C, Corwin J, Tsoukalas A, Zhang L, Ware D, Pauly M, Kliebenstein DJ, Dehesh K, Tagkopoulos I, Breton G, Pruneda-Paz JL, Ahnert SE, Kay SA, Hazen SP, Brady SM. Nature 517(7536):571-5

Expression of heterologous xyloglucan xylosyltransferases in Arabidopsis to investigate their role in determining xyloglucan xylosylation substitution patterns. Mansoori N, Schultink A, Schubert J, Pauly M. Planta167(4):1271-83.

COBRA-LIKE 2, a member of the GPI-anchored COBRA-LIKE family, plays a role in cellulose deposition in Arabidopsis seed coat mucilage secretory cells. Ben-Tov D, Abraham Y, Stav S, Thompson K, Loraine A, Elbaum R, De Souza A, Pauly M, Kieber JJ, Harpaz-Saad S. Plant Physiology 167 (3) :711-24.


Identification and functional characterization of the distinct plant pectin esterases PAE8 and PAE9 and their deletion mutants. de Souza A, Hull PA, Gille S, Pauly M. Planta 240(5): 1123-38. Adobe PDF icon PDF

The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter family of Arabidopsis. Rautengarten C, Ebert B, Moreno I, Temple H, Herter T, Link B, Doñas-Cofré D, Moreno A, Saéz-Aguayo S, Blanco F, Mortimer JC, Schultink A, Reiter WD, Dupree P, Pauly M, Heazlewood JL, Scheller HV, Orellana A. PNAS 111(31):11563-8

A b-glucuronosyl-transferase from Arabidopsis thaliana involved in the biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth. Knoch E, Dilokpimol A, Tryfona T, Poulsen CP, Xiong G, Harholt J, Peterson BL, Ulvskov P, Hadi MZ, Kotake T, Tsumuraya Y, Pauly M, Dupree P, Geshi N. Plant Journal 76 (6), 1016-1029

RWA proteins play vital and distinct roles in cell wall O-acetylation in Arabidopsis thaliana. Manabe Y, Verhertbruggen Y, Gille S, Harbolt J, Chong SL, Pawar P, Mellerowicz E, Tenkanen M, Cheng K, Pauly M, Scheller HV. Plant Physiology 163, 1107-1117

Two-step delignification of Miscanthus to enhance enzymatic hydrolysis: Aqueous ammonia followed by sodium hydroxide and oxidants. Liu Z, Padmanabhan S, Cheng K, Xie H, Gokhale A, Na H, Pauly M, Bell AT, Prausnitz JM. Energy and Fuels 28, 542-548


Hemicellulose biosynthesis. M Pauly, S Gille, L Liu, N Mansoori, A de Souza, A Schultink, G Xiong. Planta 238 (4), 627-642

The Identification of Two Arabinosyltransferases from Tomato Reveals Functional Equivalency of Xyloglucan Side Chain Substituents. A Schultink, K Cheng, YB Park, DJ Cosgrove, M Pauly. Plant physiology 163 (1), 86-94

Solution-State 2D NMR Spectroscopy of Plant Cell Walls Enabled by a Dimethylsulfoxide-d 6/1-Ethyl-3-methylimidazolium Acetate Solvent. K Cheng, H Sorek, H Zimmermann, DE Wemmer, M Pauly. Analytical chemistry 85 (6), 3213-3221

Arabinosylation of a Yariv-Precipitable Cell Wall Polymer Impacts Plant Growth as Exemplified by the Arabidopsis Glycosyltransferase Mutant ray1. S Gille, V Sharma, EEK Baidoo, JD Keasling, HV Scheller, M Pauly. Molecular plant 6 (4) 1373-1375

Reduction in Xylan O-acetylation impacts xylem development and enzymatic recalcitrance as indicated by the Arabidopsis mutant tbl29. G Xiong, K Cheng, M Pauly. Molecular plant 6 (4) 1369-1372

Comparative transcriptomics in wild and domesticated tomato. Koenig D, Jimenez-Gomez JM, Kimura S, Fulop D, Chitwood DH, Headland LR, Kumar R, Covington MF, Devisetty UK, Tat AV, Tohge T, Bolger A, Schneeberger K, Ossowski S, Lanz C, Xiong G, Taylor-Teeples M, Brady SM, Pauly M, Weigel D, Usadel B, Fernie AR, Peng J, Sinha NR, Maloof JN. PNAS 110(28), 2655-2662


RNA-Seq analysis of developing nasturtium seeds (Tropaeolum majus): identification and characterization of an additional galactosyltransferase involved in xyloglucan biosynthesis. JK Jensen, A Schultink, K Keegstra, CG Wilkerson, M Pauly. Molecular plant 5 (5), 984-992

O-acetylation of plant cell wall polysaccharides. S Gille, M Pauly. Frontiers in plant science


Velasquez SM, Ricardi MM, Dorosz JG, Fernandez PV, Nadra AD, Pol-Fachin L, Egelund J, Gille S, Ciancia M, Verli H, Pauly M, Bacic A, Olsen CE, Ulvskov P, Peterson BL, Somerville C, Iusem ND, Estevez JM, 2011, O-glycosylated cell wall proteins are essential in polarized root hair growth, Science, 332, 1401-1403

Troncoso-Ponce MA, Kilaru A, Cao X, Durrett T, Fan J, Jensen J, Pauly M, Wilkerson C, Ohlrogge J, 2011, Comparative deep sequencing profiling of four developing oilseeds, in press in Plant Journal

Gille S, Kun C, Skinner ME, Liepman AH, Wilkerson C, Pauly M, 2011, Deep Sequencing of Voodoo Lily (Amorphophallus konjac): An approach to identify relevant genes involved in the synthesis of the hemicellulose glucomannan, in press in Planta

Manabe Y, Nafisi M, Verhertbruggen Y, Orfila C, Gille S, Rautengarten C, Cherk C, Marcus SE, Somerville S, Pauly M, Knox JP, Sakuragi Y, Scheller HVS, 2011, Loss-of-function mutation of reduced wall acetylation 2 in Arabidopsis leads to reduced cell wall acetylation and increased resistance to Botrytis cinerea, Plant Physiology, 155 (3), 1068-1078

Gunl M, Neumetzler L, Kraemer F, Souza A, Schultink A, Pena M, York WS, Pauly M, 2011, AXY8 encodes an a-fucosidase, underpinning the importance of apoplastic metabolism on the fine structure of plant cell wall polysaccharides, Plant Cell 23 (11), 4025-4040

Gunl M, Pauly M, 2011, AXY3 encodes a a-xylosidase that impacts the abundance and accessibility of the hemicellulose xyloglucan in Arabidopsis plant cell walls, Planta, 233 (4), 707-719

Gunl M, Kraemer FJ, Pauly M, 2011, Oligosaccharide mass profiling (OLIMP) of cell wall polysaccharides by MALDI-TOF/MS, Methods in Molecular Biology Vol 715: The Plant Cell Wall, 43-54, Editor Popper Z, Humana Press

Perrson S, Sorensen I, Moller I, Willats W, Pauly M, 2011, Dissection of the Plant Cell Wall by High Throughput Methods, Annual Plant Reviews Vol 41: Plant Polysaccharides: Biosynthesis and Bioengineering, 43-64, Editor Ulvskov P, Wiley-Blackwell


Pauly M, 2010, A blue-print of the protoplast’s dwelling, in press in Plant Physiology

Perrson S, Willats W, Pauly M, 2010, Dissection of the Plant Cell Wall by High Throughput Methods, Plant Cell Walls, Udskov editor, in press

Gunl M, Kraemer FJ, Pauly M, 2010, Oligosaccharide mass profiling (OLIMP) of cell wall polysaccharides by MALDI-TOF/MS, in press in Methods in Molecular Biology: The Plant Cell Wall, Editor Popper, Humana Press

Lopes FJF, Pauly M, Lau EY, Diola V, Passos JL, Loureiro ME, Brommonshenkel SH, 2010, The EgMUR3 xyloglucan galactosyltransferase from Eucalyptus grandis complements the mur3 cell wall phenotype in Arabidopsis thaliana, in press in Tree Genetics and Genomes

Suen G, Scott  JJ, Aylward FO, Adams SM, Tringe SG, Pinto-Tomas AA, Foster CE, Pauly M, Weimer PJ, Barry KW, Goodwin LA, Bouffard P, Li L, Osterberger J, Harkins TT, Slater SC, Donohue TJ, Currie CR, 2010, An insect herbivore microbiome with high plant biomass degrading capacity, in press in PLoS Genetics Adobe PDF icon[PDF]

Kaida R, Serada S, Norioko N, Norioka S, Neumetzler L, Pauly M, Sampedro J, Zarra I, Hayashi T, Kaneko TS, 2010, Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells, Plant Physiology, 153 (2), 603-610 [PubMed

Gunl M, Gille S, Pauly M, 2010, OLIigo Mass Profiling (OLIMP) of extracellular polysaccharides, in production in Journal of visualized Experiments Journal of visualized Experiments, 40, doi: 10.3791/2046 [link to JoVE] Adobe PDF icon[PDF]

Pauly M, Keegstra K, 2010, Plant cell walls as precursors for biofuels, Current Opinion in Plant Biology, 13 (3), 305-312 [PubMed

Santoro N, Cantu SL, Tornqvist CEI, Falbel TG, Bolivar JL, Patterson SE, Pauly M, Walton JD, 2010, A high throughput platform for screening milligram quantities of plant biomass for lignocellulosic digestability, BioEnergy Research, 3 (1), 93-102

Foster CE, Martin T, Pauly M, 2010, Comprehensive compositional analysis of Plant Cell Walls (lignocellulosic biomass); Part II: carbohydrates, Journal of visualized Experiments, 37, doi: 10.3791/1837 [link to JoVE] Adobe PDF icon[PDF]

Foster CE, Martin T, Pauly M, 2010, Comprehensive compositional analysis of Plant Cell Walls (lignocellulosic biomass); Part I: lignin, Journal of visualized Experiments, 37; doi: 10.3791/1745 [link to JoVE] Adobe PDF icon[PDF]


Gille S, Haensel U, Ziemann M, Pauly M, 2009, Identification of plant cell wall mutants by means of a forward chemical genetic approach using hydrolases, PNAS, 106 (34), 14699-14704, Adobe PDF icon[PDF]

Obel N, Erben V, Schwarz T, Kuehnel S, Fodor A, Pauly M, 2009, Microanalysis of plant cell wall polysaccharides, Molecular Plant, 2 (5), 922-932 [PubMed]


Pauly M, Keegstra K, 2008, Tear down this wall, Current Opinion in Plant Biology 11 (3), 233-235 [PubMed

Cavalier DM, Lerouxel O, Neumetzler L, Yamauchi K, Reinecke A, Freshour G, Zabotina O, Hahn MG, Burgert I, Pauly M, Raikhel N, Keegstra K, 2008, Disruption of two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component, Plant Cell 20:1519-1537 Adobe PDF icon[PDF]

Jensen J, Sorensen S, Harholt J, Geshi N, Sakuragi Y, Moller I, Zandleven J, Bernal AJ, Jensen NB, Sorensen C, Pauly M, Beldman G, Willats WGT, Scheller HV, 2008, Identification of a xylogalactuonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis, Plant Cell 20: 1289-1302   Adobe PDF icon[PDF]

Wen F, Rhodesia MC, Nguyen T, Zeng, W, Keegstra K, Immerzeel P, Pauly M, Hawes MC, 2008, Inducible expression of Pisum sativum xyloglucan fucosyltransferase in the pea root cap meristem, and effects of antisense mRNA expression on root cap cell wall structural integrity, Plant Cell Reports 27:1125-1135 Adobe PDF icon[PDF]

Pauly M, Keegstra K, 2008, Cell wall carbohydrates and their modification as raw materials for biofuels, Plant Journal 54, 559-568 [PubMed

Leboeuf E, Immerzeel P, Gibon, Y, Steup M, Pauly M, 2008, High throughput functional assessment of polysaccharide-active enzymes using MALDI-TOF mass spectrometry as exemplified on plant cell wall polysaccharides, Analytical biochemistry, 373 (1):9-17 [PubMed]  


Bernal AJ, Jensen JK, Harholt J, Sorensen S, Moller I, Blaukopf C, Johansen B, de Lotto R, Pauly M, Scheller HV, Willats WGT, 2007, Disruption of AtCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis, Plant Journal, 52, 791-802  [PubMed

Rösti J, Barton CJ, Albrecht S, Dupree P, Pauly M, Findlay K, Roberts K, Seifert GJ, 2007, UDP-glucose 4 epimerase isoforms UGE2 and UGE4 cooperate in providing UDP-galactose for cell wall biosynthesis and growth of Arabidopsis thaliana, Plant Cell 19:1565-1579 Adobe PDF icon[PDF]

Egelund J, Obel N, Ulvskov P, Geshi N, Pauly M, Bacic A, Peterson BL, 2007, Molecular characterization of two Arabidopsis thaliana glycosyltransferase mutants, rra1 and rra2, which have a reduced residual arabinose content in a polymer tightly associated with the cellulosic wall residue, Plant Molecular Biology 64(4): 439-451 [PubMed]  

Krupkova E, Immerzeel P, Pauly M, Schmülling T, 2007, The tumorous shoot development2 gene of Arabidopsis encoding a putative methyltransferase is required for cell adhesion and co-ordinated plant development, Plant Journal 50: 735-750 [PubMed]

Kannangara R, Branigan C, Liu Y, Penfield T, Rao V, Mouille G, Höfte H, Pauly M, Riechmann JL, Broun P, 2007, Transcription factor WIN1/SHN1 regulates cutin biosynthesis in Arabidopsis thaliana, Plant Cell 19: 1278-1294 Adobe PDF icon[PDF]

Lionetti V, Railola A, Camardella L, Giovane A, Obel N, Pauly M, Favaron F, Cervone F, Bellincampi D, 2007, Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea, Plant Physiology 143:1871-1880 Adobe PDF icon[PDF]

Obel N, Neumetzler L, Pauly M, 2007, Hemicelluloses and cell expansion, in The expanding cell, Springer publishing, Verbelen C, Vissenberg K eds. p 57-88 


Carrari F, Baxter C, Usadel B, Urbanczyk-Wochniak E, Zanor MI, Nunes-Nesi A, Nikiforova V, Centero D, Ratzka A, Pauly M, Sweetlove LJ, Fernie AR, 2006, Integrated analysis of metabolite and transcript levels reveals the metabolic shifts that underlie tomato fruit development and highlight regulatory aspects of metabolic network behavior, Plant Physiology 142 (4):1380-1396 Adobe PDF icon[PDF]

Bosca S, Barton CJ, Taylor NG, Ryden P, Neumetzler L, Pauly M, Roberts K, Seifert GJ, 2006, Interactions between MUR10/CesA7-dependent secondary cellulose biosynthesis and primary cell wall structure. Plant Physiology 142:1353-1363. Adobe PDF icon[PDF]

Immerzeel P, Pauly M, 2006, Profiling methods for the analysis of cell wall polysaccharides, New Zealand Journal of Forestry research, 36 (1):145-156 

Mouille G, Witucka-Wall H, Bryant MP, Loudet O, Rihouey C, Lerouxel O, Lerouge P, Hoefte H, Pauly M, 2006, QTL analysis of primary cell wall composition in Arabidopsis thaliana, Plant Physiology, 141 (7):1035-1044Adobe PDF icon[PDF

Diet A, Link B, Seifert GJ, Schellenberg B, Wagner U, Pauly M, Reiter WD, Ringli C, 2006, The Arabidopsis root hair cell wall formation mutant lrx1 is suppressed by mutations in the RHM1 gene encoding a UDP-L-rhamnose synthase, Plant Cell, 18, 1630-1641 Adobe PDF icon[PDF

Harholt J, Jensen JK, Sorensen SO, Orfila C, Pauly M, Scheller HV, 2006, Arabinan deficient 1 is a putative arabinosyltransferase involved in biosynthesis of pectin arabinan in Arabidopsis, Plant Physiology, 140 (1): 49-58 Adobe PDF icon[PDF]

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renau JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D, 2006, A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of non redundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansin and plant growth, Plant Physiology, 140 (1), 30-48 Adobe PDF icon[PDF]

Obel N, Erben V, Pauly M, 2006, Functional wall glycomics through oligosaccharide mass profiling, in The Science and Lore of the Plant Cell Wall: Biosynthesis, Structure and Function, Hayashi, T., ed., Brownwater press, 258-266 [PubMed]


Fettke J, Poeste S, Eckermann N, Thissen A, Pauly M, Geigenberger P, Steup M, 2005, Analysis of cytosolic heteroglycans from leaves of transgenic potato (Solanum tuberosum L) plants that under- or overexpress the pho2 phosphorylase isozyme, Plant and Cell Physiology, 46 (12), 1987-2004 [PubMed]

Gibeaut DM, Pauly M, Bacic A, Fincher GB, 2005, Changes in the cell wall polysaccharides in developing barley coleoptiles, Planta, 221 (5):720-738 

Kanter U, Usadel B, Guerinaeu F, Li Y, Pauly M, Tenhaken R, 2005, The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell wall matrix polysaccharides, Planta, 221 (2), 243-254 [PubMed]

Usadel B, Kuschinsky AM, Steinhauser D, Pauly M, 2005, Transcriptional co-response analysis as a tool to identify new components of the wall biosynthetic machinery, Plant Biosystems, 139(1), 69-73 

Vissenberg K, Fry SC, Pauly M, Höfte H, Verbelen H, 2005, XTH acts at the microfibril-matrix interphase during cell elongation, Journal of Experimental Botany, 56 (412): 673-683 Adobe PDF icon[PDF]